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Technical Reference SpermMobil
GM501 SpermMobil
- HEPES and bicarbonate buffered
- Dilute 1:20 with sperm processing medium
- For in-vitro examination of necrozoospermic ejaculates and immotile spermatozoa isolated from testicular tissue
- Contains Theophylline
- Can be used safely for up to 7 days after opening when stored at 2-8°C and sterile conditions are maintained
- A Certificate of Analysis for each batch is available at hamiltonthorne.com
GM501 SpermMobil Intended Use
GM501 SpermMobil is a HEPES buffered, HSA free reagent containing low bicarbonate. It is used for in-vitro examination of sperm cells of necrozoo-spermic ejaculates as well as of immotile sperms isolated from testicular tissue (TESE).
*GM501 SpermMobil is for non-clinical, research use only in USA.
GM501 SpermMobil Instructions for Use
- Warm to 37°C prior to use. GM501 SpermMobil is HEPES buffered. Incubation of GM501 SpermMobil in a CO2-incubator will lower the pH.
- To facilitate sperm activation, add 1.5-2.0 μl of GM501 SpermMobil to sperm preparation-medium in the Petri-dish (approximately 30-40 μl/ dilution 1:20).
- GM501 SpermMobil should be added to the opposing side from where the sperms are to be aspirated.
- Wait for 10 minutes. The activating effect will initiate after a few minutes and lasts for up to one hour.
- Any laboratory procedures described herein are recommendations only. Each laboratory must establish and validate its own procedures for preparation and use.
GM501 SpermMobil Composition
- NaCl, KCl, KH2PO4, MgSO4 CaCl2
- Bicarbonate, HEPES, EDTA
- Glucose, Lactate, Pyruvate
- Non-essential and essential Amino Acids, Alanyl-Glutamine
- Theophylline, Phenol Red
GM501 SpermMobil Specifications
| pH (at 37°C) | 7.20 - 7.60 |
| Osmolality (mOsm/kg) | 280 - 320 |
| Sterility (sterile/SAL 10-3) | No Growth |
| Endotoxins (LAL, EU/ml) | < 0.25 |
| Human Sperm Survival Assy (HSSA) | ≥ 80% of Control Motility at 24h |
GM501 SpermMobil Order Codes
| Part # | Size | Storage | Shelf Life* |
| 4 GM 501SMOBIL5 | 1 x 5 ml | 2 - 8°C | 6 months |
| 4 GM 501SMOBIL5-S | 1 x 1ml | 2 - 8°C | 6 months |
| * From the date of manufacture | |||
GM501 SpermMobil Reference
Sandi-Monroy NL, Musanovic S, Zhu D, et al. Use of dimethylxanthine theophylline (SpermMobil®) does not affect clinical, obstetric or perinatal outcomes. Arch Gynecol Obstet. 2019; 300(5): 1435-1443. doi:10.1007/s00404-019-05312-8.
MKT-2016 Rev. A, 06-Nov.-2020| File | Description | File size |
|---|---|---|
 MKT-2016 GM501 SpermMobil | GM501 SpermMobil Brochure | 1820 kB |
| GM501 SpermMobil GYN IFU.pdf | GM501 SpermMobil Instructions for Use | 3098 kB |