SpermActive

GM501 SpermActive

Ready to use
HEPES (15mM) and bicarbonate buffered
Suitable for washing, swim-up and density gradient centrifugation
For handling of testicular tissue
Contains Gentamicin (10 mg/liter) and Phenol Red
Includes highest available purity (Ph. Eu./USP) Human Serum Albumin (5.00 g/liter)
Can be used safely for up to 7 days after opening when stored at 2 - 8°C and sterile conditions are maintained
A Certificate of Analysis for each batch is available on hamiltonthorne.com.
Available in 20ml and 50ml sizes

GM501 SpermActive Intended Use

GM501 SpermActive is intended for the handling and preparation of sperm for use in assisted reproduction procedures. GM501 SpermActive is not intended for use in intrauterine insemination (IUI) procedures.

GM501 SpermActive is a ready-to-use medium designed for sperm washing, swim-up techniques and density gradient centrifugation, as well as for testicular tissue.

GM501 SpermActive Instructions for Use

Prior to use, GM501 SpermActive must be equilibrated overnight in a humidified CO₂-incubator (at 5 - 7% CO₂, 37°C).
Any laboratory procedures described herein are recommendations only. Each laboratory must establish and validate its own procedures for preparation and use.

GM501 SpermActive Composition

NaCl, KCl, KH₂PO₄, MgSO, CaCl₂
Bicarbonate, HEPES, EDTA
Glucose, Lactate, Pyruvate
Non-essential and essential Amino Acids, Alanyl-Glutamine
Human Serum Albumin, Gentamicin, Phenol Red

GM501 SpermActive Specifications

pH (at 37°C, 6%CO₂)	7.20 - 7.50
Osmolality (mOsm/kg)	270 - 290
Sterility (sterile/SAL 10^-3)	No Growth
Endotoxins (LAL, EU/ml)	< 0.25
Human Sperm Survival Assy (HSSA)	≥ 80% of Control Motility at 24h

GM501 SpermActive Order Codes

Part #	Size	Storage	Shelf Life*
4 GM501SA-020	1 x 20ml	2 - 8°C	6 months
4 GM501SA-050	1 x 50ml	2 - 8°C	6 months
		* From the date of manufacture

MKT-2011 Rev. A, 06-Nov.-2020

Attachments:
File	Description	File size
MKT-2011 GM501 SpermActive Rev.A	GM501 SpermActive Brochure	1806 kB