GM508 CultActive

GM508 CultActiveCultActive edited no CE mark

  • Bicarbonate buffered
  • Ready to use
  • For investigation of fertilization failure (deficient oocyte activation)
  • Contains Ca2+-Ionophore A23187
  • A Certificate of Analysis for each batch is available at hamiltonthorne.com

GM508 CultActive Intended Use

GM508 CultActive is a bicarbonate buffered, HSA free reagent designed to investigate oocytes of pa­tients with failed fertilization after previous intracy­toplasmatic sperm injection (ICSI) cycles.

GM508 CultActive is designed to investigate if fer­tilization failure after previous ICSI cycles is due to a deficient oocyte activation.

*GM508 CultActive is for non-clinical, research use only in USA.

GM508 CultActive Instructions for Use

  • Prior to use, both GM508 CultActive and culture media (i.e.,GM501 Cult) must be equilibrated for 4 hours in vial not firmly closed at 5 - 7% CO₂ and 37°C prior to use.
  • Any laboratory procedures described herein are recommendations only. Each laboratory must establish and validate its own procedures for preparation and use.
  • See below for recommended application.

GM508 CultActive Composition

  • NaCl, KCl, KH2PO4, MgSO4 CaCl2
  • Bicarbonate, EDTA
  • Glucose, Lactate, Pyruvate
  • Non-essential and essential Amino Acids, Alanyl-Glutamine
  • Ca2+-Ionophore A23187, DMSO

GM508 CultActive Specifications

pH (at 37°C, 6%CO2) 7.00 - 7.60
Osmolality (mOsm/kg) 400 - 460
Sterility (sterile/SAL 10-3)  No Growth
Endotoxins (LAL, EU/ml) < 0.25
1-cell MEA ≥ 80% blastocysts at 96h after
1h exposure to GM508 CultActive


GM508 CultActive Order Codes

Part # Size Storage Shelf Life*
 4 GM 508CULT-active1  1 x 1 ml 2 - 8°C

6 months 

      * From date of manufacture

CultActive References

Murugesu S, Saso S, Jones BP, et al. Does the use of calcium ionophore during artificial oocyte activation demonstrate an effect on pregnancy rate? A meta-analysis. Fertil Steril. 2017;108(3):468-482.e3. doi:10.1016/j.fertnstert.2017.06.029

Ebner T, Montag M; Oocyte Activation Study Group, et al. Live birth after artificial oocyte activation using a ready-to-use ionophore: a prospective multicentre study. Reprod Biomed Online. 2015;30(4):359-365. doi:10.1016/j.rbmo.2014.11.012

 
MKT-2015 Rev. A, 06-Nov-2020
Attachments:
FileDescriptionFile size
Download this file (MKT-2015-GM508-CultActive-RevA-Nov-06-2020-Final.pdf)MKT-2015 GM508 CultActive Rev.AGM508 CultActive Brochure 1666 kB
Download this file (GM508 CultActive GYN IFU.pdf)GM508 CultActive GYN IFU.pdfGM508 CultActive Instructions for Use3401 kB