- Bicarbonate buffered
- Ready to use
- For investigation of fertilization failure (deficient oocyte activation)
- Contains Ca2+-Ionophore A23187
- A Certificate of Analysis for each batch is available at hamiltonthorne.com
GM508 CultActive Intended Use
GM508 CultActive is a bicarbonate buffered, HSA free reagent designed to investigate oocytes of patients with failed fertilization after previous intracytoplasmatic sperm injection (ICSI) cycles.
GM508 CultActive is designed to investigate if fertilization failure after previous ICSI cycles is due to a deficient oocyte activation.
*GM508 CultActive is for non-clinical, research use only in USA.
GM508 CultActive Instructions for Use
- Prior to use, both GM508 CultActive and culture media (i.e.,GM501 Cult) must be equilibrated for 4 hours in vial not firmly closed at 5 - 7% CO₂ and 37°C prior to use.
- Any laboratory procedures described herein are recommendations only. Each laboratory must establish and validate its own procedures for preparation and use.
- See below for recommended application.
GM508 CultActive Composition
- NaCl, KCl, KH2PO4, MgSO4 CaCl2
- Bicarbonate, EDTA
- Glucose, Lactate, Pyruvate
- Non-essential and essential Amino Acids, Alanyl-Glutamine
- Ca2+-Ionophore A23187, DMSO
GM508 CultActive Specifications
|pH (at 37°C, 6%CO2)||7.00 - 7.60|
|Osmolality (mOsm/kg)||400 - 460|
|Sterility (sterile/SAL 10-3)||No Growth|
|Endotoxins (LAL, EU/ml)||< 0.25|
|1-cell MEA||≥ 80% blastocysts at 96h after
1h exposure to GM508 CultActive
GM508 CultActive Order Codes
|Part #||Size||Storage||Shelf Life*|
|4 GM 508CULT-active1||1 x 1 ml||2 - 8°C||
|* From date of manufacture|
Murugesu S, Saso S, Jones BP, et al. Does the use of calcium ionophore during artificial oocyte activation demonstrate an effect on pregnancy rate? A meta-analysis. Fertil Steril. 2017;108(3):468-482.e3. doi:10.1016/j.fertnstert.2017.06.029
Ebner T, Montag M; Oocyte Activation Study Group, et al. Live birth after artificial oocyte activation using a ready-to-use ionophore: a prospective multicentre study. Reprod Biomed Online. 2015;30(4):359-365. doi:10.1016/j.rbmo.2014.11.012MKT-2015 Rev. A, 06-Nov-2020