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GM508 CultActive

GM508 CultActive is a bicarbonate buffered, HSA free reagent designed to investigate oocytes of pa­tients with failed fertilization after previous intracy­toplasmatic sperm injection (ICSI) cycles.

GM508 CultActive is designed to investigate if fer­tilization failure after previous ICSI cycles is due to a deficient oocyte activation.

*GM508 CultActive is for non-clinical, research use only in USA.

Specifications

  • Bicarbonate buffered
  • Ready to use
  • For investigation of fertilization failure (deficient oocyte activation)
  • Contains Ca2+-Ionophore A23187
  • A Certificate of Analysis for each batch is available at hamiltonthorne.com
pH (at 37°C, 6%CO2)7.00 – 7.60
Osmolality (mOsm/kg)400 – 460
Sterility (sterile/SAL 10-3) No Growth
Endotoxins (LAL, EU/ml)< 0.25
1-cell MEA≥ 80% blastocysts at 96h after
1h exposure to GM508 CultActive

Composition

  • NaCl, KCl, KH2PO4, MgSO4 CaCl2
  • Bicarbonate, EDTA
  • Glucose, Lactate, Pyruvate
  • Non-essential and essential Amino Acids, Alanyl-Glutamine
  • Ca2+-Ionophore A23187, DMSO

Instructions for Use

Prior to use, both GM508 CultActive and culture media (i.e.,GM501 Cult) must be equilibrated for 4 hours in vial not firmly closed at 5 – 7% CO₂ and 37°C prior to use.

Any laboratory procedures described herein are recommendations only. Each laboratory must establish and validate its own procedures for preparation and use.

Recommended Application

  • Prior to use, both GM508 CultActive and culture medium (i.e. GM501 Cult) must be equilibrated for 4 hours at 5-7% CO and 37°C.
  • Prior to equilibrating, remove vial stopper and loosely replace to allow vial to vent.
  • Shake GM508 CultActive for approximately 30 seconds immediately prior to use.
  • For each oocyte, prepare 1 drop (30 μl) GM508 CultActive (1# in diagram below) and 2 drops  (30-50 μl) MOPS-and HEPES-free culture medium (#2 and #3 in diagram below). An oil overlay is not necessary due to the short exposure time.
  • Immediately after the ICSI procedure, incubate the oocytes for 15 minutes in the equilibrated CA²⁺-Ionophore GM508 CultActive drops (#1).
  • Remove the oocytes from the GM508 CultActive drop and wash them twice in a HEPES-or MOPS-free culture medium (#2 and #3).
  • Place the oocytes in culture media for further culture.
  • Assess the development at desired time points. 
  • Any laboratory procedures described herein are recommendations only. Each laboratory must establish and validate its own procedures for preparation and use. 

Resources

Part Number

4 GM 508CULT-active1

Size

1 x 1 ml

Storage

2 – 8°C

Shelf Life

6 months

*From the date of manufacture

Murugesu S, Saso S, Jones BP, et al. Does the use of calcium ionophore during artificial oocyte activation demonstrate an effect on pregnancy rate? A meta-analysis. Fertil Steril. 2017;108(3):468-482.e3. doi:10.1016/j.fertnstert.2017.06.029

Ebner T, Montag M; Oocyte Activation Study Group, et al. Live birth after artificial oocyte activation using a ready-to-use ionophore: a prospective multicentre study. Reprod Biomed Online. 2015;30(4):359-365. doi:10.1016/j.rbmo.2014.11.012