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CEROS II

The CEROS II is a highly accurate sperm analyzer that is a cost-effective alternative to our industry leading IVOS CASA system (Computer Assisted Sperm Analysis). Perform semen quality assessment for sperm count and concentration; motile categories like static, motile, progressive. View detailed kinematic values for each cell and the kinematic profiles for the entire population. For over 30 years, Hamilton Thorne has been helping IVF professionals and animal researchers obtain sperm analysis results for applications in multiple markets. Our CEROS II CASA system provides precise, objective, and repeatable results that you can trust. 

  • Unique color-coded illumination to optimize identification of the sperm head and tail 
  • Cost-effective and space-saving sperm analyzer with external phase contrast microscope included  
  • Additional software modules for DNA Fragmentation, Morphology  

*availability of software modules various per application

  • Human
  • Animal

Applications

Not for clinical diagnostic procedures in USA – for research purposes only

Performing semen analysis is essential for evaluating male factor infertility in IVF applications. Our CEROS II sperm analyzer provides an efficient method for performing semen assessment in the lab. 

  • Semen Analysis for counts, motility, progressive, concentration, volume 
  • Sperm Functional Tests 
  • DNA Fragmentation with SCD assay (Sperm Chromatin Dispersion Test) 
  • Sperm Morphology

Specifications

CEROS II Microscope: ZEISS Axio lab A5
Objectives Standard: 10x negative or positive phase, 20x brightfield, 100x brightfield  
Image Type Negative or Positive Phase Contrast
Brightfield
Temperature Control Minitherm stage warmer available as an accessory for temperature control.
Compatible Specimen Chambers Type: 20 micron depth slide 
Image Acquisition Frame Rate: 60 Hz 
Frames: 1 – 60 
Fields: 1 – 100 Field Selection: Manual 
Power Requirements Input voltage: 110-240 VAC Power: 250 watt (max) Line Frequency: 50/60 Hz 

Features & Benefits

The CEROS II sperm analyzer provides the same analysis software as the IVOS II at a cost-effective price point and space-saving footprint in your lab. It is ideal for lower volume throughput labs, and groups that maintain strict operating procedures surrounding equipment and higher skill set technicians. For high volume and high turn over labs with lower skill set requirements, the automated IVOS may be more ideal for your setting.

Spend less time on learning a new system with the CEROS II’s intuitive Windows-based software platform and standard illumination controls.  

Software

The CEROS II sperm analyzer is powered by our user-friendly HT CASA software. Analyze immotile, motile, progressive, slow cell, and total concentration using WHO 4, 5, or 6 parameters. 

Color-coded Parameters and Interactive Illumination Check 
  • Motile sperm identified by color-coded tracks and static sperm identified by red dot. 
  • Unique color-coded Illumination helps optimize identification of the sperm head and tail.  
  • Motility track overlay on playback image and results may be visible or turned off.
Analysis Results Updated in Real Time  
  • WHO Standards and Analysis Results are updated in real-time as each field is added.
  • Captured fields with motility tracks are displayed in video thumbnail gallery.
  • Selecting a field from thumbnail gallery displays summary counts and kinematics.
  • Each track can be selected and zoomed to display detailed results.
  • Results can be saved to the database.
Customized Reports
  • Analysis results can be exported via ASCII file output.  
  • Display sperm analysis results with user-friendly drag and drop report designer. 
  • Unlimited number of customized reports including analysis images.
Replicate Analysis
  • Perform replicate analysis following WHO guidelines between two different chambers
  • Determine if the replicate samples are within acceptable difference by calculating the highest average of cells within the motile category.
  • Perform calculations automatically within the software.
Internal Quality Control Modules  
  • Internal Quality Control (IQC) feature verifies microscope objective calibration daily.
  • Using standardized quality materials of known concentrations, the IQC system tracks daily performance results before initiating patient samples.
  • Determine if the replicate samples are within acceptable difference by calculating the highest average of cells within the motile category. 
  • Findings are automatically tested to the Westgard Rules  integrated into the software to provide Pass/Fail results for analytical runs with QC material. Results are displayed in a Levey-Jennings chart.

Optional Software Modules

Morphology  

The DIMENSIONS II Morphology software automatically classifies the morphology of fixed stained human sperm samples according to the Tygerberg strict criteria.  

  • Obtain objective, repeatable results when analyzing sperm for normal, sub-normal, or abnormal morphology.
  • Display TZI (Teratozoospermia Index) results: The total number of defect points encountered during an analysis, divided by the number of Abnormal and Sub-normal cells.
  • Classify sperm by morphological characteristics of the sperm head size and shape, acrosome, and tail. 
  • Compatible with Diff-Quick or Papanicolaou (PAP) stains.  
DNA Fragmentation  

The DNA Fragmentation Software evaluates the level of DNA integrity from sperm smears prepared with the Halosperm® G2 test kit from Halotech® DNA based on the Sperm Chromatin Dispersion (SCD) Test (Fernandez et al., J. Androl 24:59-66,2003; Fertil Steril 84:833-842,2005). 

  • Analyzes prepared sperm samples and measures the size of the halo (large halo, medium halo, small halo, or no halo). 
  • Displays the number and percentage of sperm showing a Large Halo, Medium Halo, Small Halo or No Halo
  • Categorizes cells as normal, fragmented, or degraded.
  • Results are shown per selected field and per selected cell. 

Resources

Technical Support & Service Plans

Hamilton Thorne’s CASACare™ service plans offer economical and comprehensive coverage against unexpected repair costs and downtime. Two levels of service plans – Basic and Plus – are available for our CASA systems. The tiered plans give customers the flexibility to choose the features that best suit their needs. 

Applications

Performing semen analysis is essential for various animal research studies and animal breeding in farm-animal systems or veterinary care settings. Our CEROS II sperm analyzer provides an efficient method for performing semen assessment in the lab. 

  • Animal Breeding 
  • Animal Conservation  
  • Animal Production 
  • Animal Research 
  • Transgenics Research 

Specifications

CEROS II Microscope: ZEISS Axio lab A5 microscope 
Objectives Standard: 10x negative phase
Optional: 4x dark field, 20x brightfield, 40x brightfield, 60x brightfield, 100x brightfield 
Image Type Negative Phase Contrast
Brightfield, Darkfield 
Temperature Control Minitherm stage warmer available as an accessory for temperature control.
Compatible Specimen Chambers Type: 20micron depth Slide for breeder species motility analysis, 80-100micron depth slide for rodent analysis
Image Acquisition Frame Rate: 60 Hz 
Frames: 1 –60
Field Selection: Manual 
Power Requirements Input voltage: 110-240 VAC Power: 250 watt (max) Line Frequency: 50/60 Hz 

Features & Benefits

The CEROS II sperm analyzer provides the same analysis software as the IVOS II at a cost-effective price point and space-saving footprint in your lab. It is ideal for lower volume throughput labs, and groups that maintain strict operating procedures surrounding equipment and higher skill set technicians. For high volume and high turn over labs with lower skill set requirements, the automated IVOS may be more ideal for your setting.

Spend less time on learning a new system with the CEROS II’s intuitive Windows-based software platform and standard illumination controls.

Software

The CEROS II sperm analyzer is powered by our user-friendly HT CASA software. Perform semen quality assessment for sperm count and concentration, motile categories such as static, motile, progressive, slow cell, as well as all detailed kinematic values for each cell and the kinematic profiles for the entire population. For breeding applications, dosing calculations are made automatically.

Animal modules for HT CASA software: 

  • HT CASA Animal Motility- suitable for animal research applications, transgenic animal models, universities, developmental biology, and more!  Data view focused on research parameters. Choose the specific parameters for your animal study with the software’s unlimited user-defined analysis setups. Contains additional caudal counts.  
  • HT CASA Animal Breeder-suitable for animal breeding facilities, veterinarians, universities, farm animal studies, and animal production. Contains additional dosing parameters, a fully automated and customizable tail morphometry detection*, and scoring of doses based on users’ category of normal or abnormal. Defect detection includes: proximal droplets, distal droplets, bent tails, coiled tails, and Distal Midpiece Reflex. *Species and media specific by optical clarity
  • HT CASA Boar Breeder-software specific for swine breeding facilities with dose criteria and calculations for boar semen. Contains additional dosing parameters with fully automated and customizable tail morphometry detection and dose scoring based on users’ definition of normal or abnormal. Defect detection includes: proximal droplets, distal droplets, bent tails, coiled tails, and Distal Midpiece Reflex.
  • HT CASA Equine Breeder- software specific for equine breeding facilities with dose criteria and calculations for stallion semen. Contains additional dosing parameters to allow for individual doses or batch dose creation.

Contact our sales team for further information regarding your specific application! 

Color-coded Parameters and Interactive Illumination Check 
  • Motile sperm identified by color-coded tracks and static sperm identified by red dot. 
  • Unique color-coded Illumination helps optimize identification of the sperm head and tail.  
  • Color-overlays on the playback image and the results may be turned on or off. 
Analysis Results Updated in Real Time
  • Data in the Results panel reflect the entire population of cells analyzed. Analysis and Dosing results are updated in real-time as each field is added to the analysis. 
  • Results are saved within image and video gallery. Re-analyze using saved settings or new set of parameters.  
  • Selecting a track from the playback image opens up a zoomed image of the cell. Kinematic Measurements are relative only to the selected track. The video may be played or scrolled through frame by frame. 
Analysis Output

Simplify your research with customized parameters to meet your animal research needs! The intuitive HT CASA software provides quantitative results for the following parameters: 

  • Total, Static, Motile, Progressive, Slow 
    (Choice of billion or million) 
  • Concentrations: 
    Total, Static, Motile, Progressive, Slow 
    (Choice of billons/ml or millions/ml) 
  • Percentages: 
    %Total, %Static, %Motile, %Progressive, %Slow 
  • Doses: 
    Extender Volume, Final Volume, Adjusted Concentration, Number of Doses 
  • Kinematic Measures: 
    DAP, DSL, DCL, VAP, VSL, VCL, ALH, STR, LIN, BCF, WOB (Average, SD, Median) 
  • Morph Averages: 
    Head Length, Head Width 
    Head Perimeter, Head Area 
    Tail Length, Tail Straightness 
    Droplet Distance, Droplet Frame Count 
    Bent Tail Count, Coiled Tail Count, 
    DMR Count 
Customized Reports
  • Analysis results can be exported via ASCII file output.
  • Display sperm analysis results with user-friendly drag and drop report designer.

Optional Software Modules

Morphology

The HT Morph morphology software automatically classifies the morphology of fixed stained animal sperm samples according to the researcher’s user-defined parameters.  HT Morph assesses each sperm cell for size, shape, acrosome, tail, and cytoplasmic droplets. The measured values are then compared to the user-defined parameters for classification as normal, abnormal, or rejected cells.

  • Obtain objective, repeatable results when analyzing sperm for normal or abnormal morphology.  
  • Classify sperm by morphological characteristics of the sperm head size and shape, acrosome, and tail based on your standards. 
  • Create an unlimited number of rule sets to represent a variety of species. 
  • Review results in real-time with the ability to override system classification or delete cell from analysis. 
  • Compatible with a variety of high contrast stains, such as Stat III, Diff-Quik and Papinicoulau (PAP).

Resources

Technical Support & Service Plans

Hamilton Thorne’s CASACare™ service plans offer economical and comprehensive coverage against unexpected repair costs and downtime. Two levels of service plans – Basic and Plus – are available for our CASA systems. The tiered plans give customers the flexibility to choose the features that best suit their needs.