The VIADENT stain quickly penetrates non-viable sperm heads. By comparing the cells visible under standard and fluorescent illumination, the analyzer easily determines the live cells versus the dead cells. The sperm cells are first identified and tracked under visible illumination as with standard analysis; then, fluorescence illumination is applied for one additional frame to identify non-viable sperm.
Simply stain an aliquot of the sample with the pre-packaged VIADENT stain for 2 minutes, load into the disposable analysis chamber, and place on the IVOS II stage. You are now ready to begin analysis!
Validation of an objective approach for simultaneous assessment of viability and motility of fresh and cooled equine spermatozoa.
M.T. Wessel, G.C. Althouse
Proceedings of the International Symposium on Equine Reproduction, Animal Reproduction Science 94 (2006) 21-22
"Our results suggest that the "Viadent" option of the IVOS system is capable of a rapid, accurate and objective evaluation of both viability and motility parameters using large numbers of spermatozoa. Application of this technique in the industry may prove useful in the clinical assessment of fertilizing potential of equine spermatozoa."
|Althouse-Viadent-Equine.pdf||Validation of an objective approach for simultaneous assessment of viability and motility of fresh and cooled equine spermatozoa.||294 kB|