The VIADENT Fluorescence option is used as a method of determining viable sperm count, concentration, and percentage from a motile sample. Standard motility analysis is performed under typical phase contrast illumination, and vaibility analysis is then performed on the same fields under fluorescent illumination.

By staining the cells with the VIADENT Stain, all non-viable sperm will fluoresce under the appropriate illumination. Viable sperm cells do not take up the stain, will not fluoresce, and will not be visible under fluorescent light.

For added convenience, Hamilton Thorne sells prepackaged VIADENT Stain tubes. Sold in quantities of 200 tubes per bag, each tube contains 40 µg of stain, which is readily dissolved in a sperm suspension medium.


FileDescriptionFile size
Download this file (ViaDentInstructions.pdf)VIADENT Instructions.pdfVIADENT Instructions21 kB
Download this file (SDS-0002 (US) VIADENT Stain Rev. A.pdf)SDS-0002 (US) VIADENT Stain Rev. A.pdfUS VIADENT SDS122 kB
Download this file (SDS-0005 (EU) VIADENT Stain Rev. A.pdf)SDS-0005 (EU) VIADENT Stain Rev. A.pdfEU VIADENT SDS141 kB