XYClone is for non-clinical research use only.hESC research using the XYClone should be conducted in accordance with any applicable Federal, State or local laws and regulations.
Please Note: The XYClone is for use ONLY with ANIMAL and STEM CELL APPLICATIONS. (For Clinical Laser Assisted Hatching and Biopsy applications, please see the ZILOS-tk.)
XYClone Customer Testimonials
Oregon National Primate Reearch Center
Masahito Tachibana, Ph.D., Senior Research Associate, Oregon National Primate Research Center, Oregon Health and Science University
Prior to the XYClone laser, Tachibana was performing the nuclear transfer procedure without a laser system, which he believes “required a higher level of skill and a higher learning curve.” Tachibana says, “The XYClone laser has helped us tremendously, especially for enucleation of the spindle-chromosome complex from mature MII oocytes. In primate oocytes, it is possible to remove spindles without laser assisted zona drilling. However, it would be very difficult to stay focused on both the spindle and pipette tip (without use of the laser), because you would need to exert more force with the pipette tip to pierce through the zona pellucida; thus, causing you to lose focus of where the spindle is. Since we have started using XYClone to drill a hole in the zona pellucida, I have been able to easily focus on both the spindle and pipette tip at the same time during the whole enucleation step.” Download PDF of Testimonial
New York Stem Cell Foundation
Dieter Egli, Ph.D. Senior Research Fellow, New York Stem Cell Foundation;
Research Scientist in Pedriatics & Molecular Genetics, Columbia University
“I am using the XYClone to drill the zona pellucida of both human and mouse oocytes or zygotes. It is very fast, offers reliable zona drilling and makes nuclear transfer very convenient. The XYClone is a great tool that works well with other tools and methods, including the piezo drill. With improved objectives, it is now possible to perform all manipulations with the laser objective.” Download PDF of Testimonial
Thomas M. DeChiara, Ph.D., Sr. Director of Transgenic Technology and Research Animal Facility
William Poeymirou, Sr. Manager of the Velocigene Vivarium and Transgenic Technology
Joseph Hickey, RAIII (Microinjector)
Jennifer Escaravage, RAIII (Microinjector)
“The laser-assisted injection of 8-cell stage mouse embryos has allowed us to generate fully ES cell-derived F0 mice (VelociMice) in high yield using inbred or hybrid ES cells. Last year we generated about 200 lines of mice. The use of the XYClone Laser System has made our process much more efficient and has resulted in phenotypable F0 mice at dramatically lower mouse costs.” Thomas DeChiara
Download PDF of Testimonial
Mutant Mouse Regional Resource Center, Univeristy of North Carolina
Kathy Mohr, Facility Director
I have been able to take a process that took months and reduce it to days. I was able to transplant twice as many embryos. Download PDF of Testimonial.
Maisam 'Maya' Mitalipova, Ph.D.
Research Scientist, Rhodes Center, University of Georgia
hES Consultant, firstname.lastname@example.org
"Laser technology is very essential for isolation of human ES cells because it eliminates the use of animal product, such as antibodies for immunosurgery, and allows very precise separation of ICM from trophoblast, maintaining the intactness of inner cell mass. It is essential for xenofree ES cells." (Download PDF of testimonial)
View Copies of Some of Maya's Publications:
Schulz TC, Palmarini GM, Noggle SA, Weiler DA, Mitalipova MM, Condie BG. Directed neuronal differentiation of human embryonic stem cells. BMC Neurocsience, 2003; 4(1):27.
Mitalipova MM, Calhoun J, Shin S, Wininger D, Schulz T, Noggle S, Venable A, Lyons I, Robins A, Stice S.. Human pluripotent embryonic stem cell lines derived from discarded embryos. Stem Cells, 2003;21(5):521-526.
Dominko T., Mitalipova M., Haley B., Beyhan Z., Memili E., Mcqucik B., and First N. Bovine oocyte cytoplasm supports development of embryos produced by nuclear transfer of somatic cell nuclei from various mammalian species. Biology of Reproduction, 1999, Vol.60, pp. 1496-1502.
Senior Research Associate
University of Newcastle
Institute of Human Genetics
Department of Stem Cell Biology and Developmental Genetics
The XYClone laser has been of huge benefit to developing our technique of human somatic cell nuclear transfer. Prior to using the XYClone we were relying on traditional enucleation procedures to remove the genetic material from human oocytes. This generally requires piercing through the elastic outer protein shell of the egg by using a microscopic beveled glass pipette, which often induced damage and resulted in lysis of the egg. The elasticity of the human zona pellucida compared with other species remained a challenge. However, following installation of the XYClone laser objective, we have been able to speed the process of enucleation to a matter of seconds, without eliciting damage to the egg. The XYClone laser suitably makes a very small, neat slit in the zona pellucida which facilitates the DNA removal. We have been very impressed by how it has dramatically improved our enucleation efficiency and reduced the lysis rates of the eggs. It has also been incredibly easy to operate and we love how easy it is to target the site of interest using the software provided. It appears to be an extremely powerful tool that has improved our ability to create human cloned embryos for the purposes of deriving patient specific stem cell lines which may one day be used to treat non-curable diseases. (Download PDF of testimonial)