XYClone is for non-clinical research use only.hESC research using the XYClone should be conducted in accordance with any applicable Federal, State or local laws and regulations.
Please Note: The XYClone™ is for use ONLY with ANIMAL and STEM CELL APPLICATIONS. (For Clinical Laser Assisted Hatching and Biopsy applications, please see the ZILOS-tk.)

XYClone Specifications

Laser
Type: 1450 nm, Infrared Solid State Diode
Power: 300 mW @ focus (Class I)
Laser Pulse Length: Adjustable from 1 microsecond to 3000 microseconds, Pulse slider resolution - 1, 10, and 100 microsecond intervals
Laser Power: Adjustable from 1% to 100%
Laser Mode: Single mode standard. Optional: Double Mode
Firing: By mouse. Optional: Remote firing by foot control
Aiming Marker: Circle or arrow, adjustable “Blink Time” after firing. Isotherm Rings, showing peak temps and hole size.
Crosshairs: Activation, size and color set by user (used for positioning embryo and moving automatic stage)
Laser Alignment: Aligned and locked in place at factory. No on-site laseer alignment required.
Target Alignment: Performed interactively on-screen

Objective
Standard: 40X or 20x, L.W.D. 0.60 N.A., I.R. (High transmission in both the visible and near-infrared, long working distance); 20x objective also available
Scale Calibration: Calibrate and store multiple objectives; Calibration performed interactively on-screen

RED-i Target Locator (optional)
Source: Red LED built into laser module
Alignment: X-Y mechanical adjustment to center of laser beam using Isotherm Rings as guide
Intensity: On-screen intensity adjustment

Video
Camera: High Resolution Color Analog Optional: Digital
Input: Video capture board, internal
Screen Resolution: Desktop: 1024 x 768. Laptop: 1280 x 1024
Image Area: Desktop: 640 x 480pixels. Laptop: 1024 x 768
Zoom: 2x, 4x and 8x, with image panning (user defined increments); Laser may be fired under zoom.
Illumination: Microscope, image on screen

Laser Safety
Class I Laser: Laser pulse and energy cannot exceed Class I limits.

Image Capture
Capture and store unlimted images. Images may be stored in JPG, BMP, or TIF format. Manual and automtaic imge file naming. Image Toolbox included (add text, shapes, and measurements to any captured image)

Double Validation Panel
Set two separate power and pulse length combinations

Image Capture and Storage Utility
Capture and store unlimited images. Images stored in user-selected JPG, BMP, or TIF format. Capture unlimited thumbnail images and select which to save. Automatic image capture on laser fire. Manual or automatic file naming. Images may be saved with graphic overlay.

Real-time Video Capture
Record and store video in .avi format. Ability to set maximum recording length. Automatic and manual file naming options.

Time-lapse Video Capture
Record and store video in .avi format. Ability to define capture interval and set maximum recording length. Automatic and manual file naming options.

Documentation Features

Measurement Toolbox
Tools allow measurement of various embryo parameters on captured images. Each measure visible as graphic overlay, including length in micron
Zona: 5 zona thickness measurements; Mean & Standard Deviation
Embryo: 2 diameter measurements; Mean & Standard Deviation; Blastomere count.
Pronuclei: 2 diameter measurements for two separate pronuclei; Mean for each pronuclei
Drill: 5 hole size measurements; Mean & Standard Deviation
Ruler: 5 user-defined measurements Mean & Standard Deviation

Reports and Output
Data Input: Patient Data from keyboard; Data from measurements; Input critical fields from ASCII file
Patient Record: Patient information, treatment identifiers, before and after treatment images, embryo evaluation data
Output: Printout of Patient Record; Patient Record stored in JPG format; ASCII output of all numerical and alphanumeric fields in TXT and MER formats (IDS, Recdate compatible)

Annotation Capabilities
Add text, ellipses, rectangles, lines to captured images. Measue any area of image with ruler tool. Custom select font, color and line widths.

Compatibility
All brands/models of inverted microscopes (no laser module customization needed)
Manipulators
Fluorescence

Dimensions H x W x D in. (mm) / lb (kg)
XYClone Module: 1.65 (42) x 1.1 (28) x 2.1 (54) / 0.5 (0.22)
Controller: 3.8 (95) x 2.5 (63.5) x 1.8 (46) / 1.0 (0.45)
Desktop Computer: 16.1 (408) x 7.4 (187) x 17 (433) / 26.5 (12)
Monitor: 13.5 (343) x 17.5 (444) x 1.5 (38.1) / 17.0 (7.7)
Laptop Computer: 1.3 (3.3) x 15.3 (388) x 9.9 (251) / 6.2 (2.8)

Electrical
Input Voltage: Desktop: 100/240 VAC, Monitor: 110-240 VAC, Laptop: 100-240 VAC
Power: Desktop: 300 W, Monitor: 30 W, Laptop: 90W
Line Frequency: Desktop: 50/60 Hz, Monitor: 50/60 Hz, Laptop: 50/60 Hz

XYClone Computer Specifications (subject to change without notice)
Type Desktop Laptop
O.S. Windows 7 Professional Windows 7 Professional
CPU Pentium Dual Core 3 GHz or Core i5 Intel Core i5
Memory 2 GB DDR 4 GB
Drives

250 GB HD
16x DVD+/-RW SATA

250 GB HD
8x DVD+/-RW
Display 20" flat panel 15" (typical), XGA
Ports Serial, Parallel, USB 2.0, PS/2 Parallel, USB 2.0, VGA, 1394, HDMI, Memory card reader
Network 10/100 Mps Ethernet 10/100 Mps Ethernet
Wireless Network (802.11bg)
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