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XYClone is for non-clinical research use only.hESC research using the XYClone should be conducted in accordance with any applicable Federal, State or local laws and regulations.
Please Note: The XYClone™ is for use ONLY with ANIMAL and STEM CELL APPLICATIONS. (For Clinical Laser Assisted Hatching and Biopsy applications, please see the ZILOS-tk.)
Download PDF of XYClone Reference List (November 1, 2011)
Please email us to request reprints.
Journal Articles
2012
Masahito Tachibana M, Sparman M, Ramsey C, Ma H, Lee HS, Penedo MCT, Mitalipov S. Generation of Chimeric Rhesus Monkeys, Cell, , Available online 5 January 2012
2011
Egli D, Chen AE, Saphier G, Ichida J, Fitzgerald C, Go KJ, Acevedo N, Patel J, Baetscher M, Kearns WG, Goland R, Leibel RL, Melton DA, Eggan K. Reprogramming within hours following nuclear transfer into mouse but not human zygotes. Nat Commun. 2011 Oct 4;2:488. doi: 10.1038/ncomms1503. PubMed PMID: 21971503.
Moawad AR, Choi I, Zhu J, Campbell KH. Ovine Oocytes Vitrified at Germinal Vesicle Stage as Cytoplast Recipients for Somatic Cell Nuclear Transfer (SCNT). Cell Reprogram. 2011 Aug;13(4):289-96. Epub 2011 Jun 30. PubMed PMID: 21718110.
Noggle S, Fung HL, Gore A, Martinez H, Satriani KC, Prosser R, Oum K, Paull D, Druckenmiller S, Freeby M, Greenberg E, Zhang K, Goland R, Sauer MV, Leibel RL, Egli D. Human oocytes reprogram somatic cells to a pluripotent state. Nature. 2011 Oct 5;478(7367):70-5. doi: 10.1038/nature10397. PubMed PMID: 21979046.
Pryor JH, Looney CR, Romo S, Kraemer DC, Long CR. Cryopreservation of in vitro produced bovine embryos: effects of lipid segregation and post-thaw laser assisted hatching. Theriogenology. 2011 Jan 1;75(1):24-33. Epub 2010 Sep 15. PubMed PMID: 20833420.
Scott BR. Development and permeability of equine blastocysts. A Thesis submitted to the Graduate Faculty of the Louisiana State University and Agricultural and Mechanical College. May 2011.
Sinko I, Katona RL. Transfer of Stem Cells Carrying Engineered Chromosomes with XY Clone Laser System. Methods Mol Biol. 2011;738:183-98. PubMed PMID: 21431728.
Siripattarapravat K, Cibelli JB. "Method for somatic cell nuclear transfer in zebrafish." The Zebrafish: Genetics, Genomics and Informatics, 3rd Edition. Eds., H. William Detrich, III, Monte Westerfield, Leonard I. Zon. Methods Cell Biol. 2011;104:209-17. PubMed PMID: 21924165
Smits K, Govaere J, Hoogewijs M, Piepers S, Van Soom A. A Pilot Comparison of Laser-Assisted vs Piezo Drill ICSI for the In Vitro Production of Horse Embryos. Reprod Domest Anim. 2011 Sep 24. doi: 10.1111/j.1439-0531.2011.01814.x. [Epub ahead of print] PubMed PMID: 21950451.
Wolf XA, Klein T, Garcia R, Hyttel P, Serup P. Identification of a conserved cis-acting region driving expression of mouse Eomesodermin to the primitive streak, node, and definitive endoderm. Gene Expr Patterns. 2011 Jul 8. [Epub ahead of print] PubMed PMID: 21763783.
2010
Aguilar-Gallardo C, Poo M, Gomez E, Galan A, Sanchez E, Marques-Mari A, Ruiz V, Medrano J, Riboldi M, Valbuena D, Simon C. Derivation, characterization, differentiation, and registration of seven human embryonic stem cell lines (VAL-3, -4, -5, -6M, -7, -8, and -9) on human feeder. In Vitro Cell Dev Biol Anim. 2010 Apr;46(3-4):317-26. Epub 2010 Feb 26. PubMed PMID: 20186513.
Alliegro MA, Henry JJ, Alliegro MC. Rediscovery of the nucleolinus, a dynamic
RNA-rich organelle associated with the nucleolus, spindle, and centrosomes. Proc
Natl Acad Sci U S A. 2010 Aug 3;107(31):13718-23. Epub 2010 Jul 19. PubMed PMID:
20643950.
Chiasson M. Laser-assisted zona pellucida hatching of frozen-thawed In vivo-produced bovine embryos. A Thesis submitted to the Graduate Faculty of the Louisiana State University and Agricultural and Mechanical College. December 2010.
Choi I, Campbell KH. Treatment of ovine oocytes with caffeine increases the accessibility of DNase I to the donor chromatin and reduces apoptosis in somatic cell nuclear transfer embryos. Reprod Fertil Dev. 2010;22(6):1000-14. PubMed PMID: 20591334.
DeChiara TM, Poueymirou WT, Auerbach W, Frendewey D, Yancopoulos GD, Valenzuela DM. Producing fully ES cell-derived mice from eight-cell stage embryo injections. Methods Enzymol. 2010;476:285-94. PubMed PMID: 20691872.
Estrada J, Li P, Mir B. Multiorgan Engraftment of Human Somatic Cells in Swine Foetuses After Intra-Blastocyst Transplantation. Reprod Domest Anim. 2010 Nov 23. doi: 10.1111/j.1439-0531.2010.01718.x. [Epub ahead of print] PubMed PMID:21092066.
Sambasiva Rao B, Uma Mahesh Y, Lakshmikantan UR, Suman K, Venu Charan K, Shivaji S. Developmental Competence of Oocytes Recovered from Postmortem Ovaries of the Endangered Indian Blackbuck (Antilope cervicapra). J Reprod Dev. 2010 Aug 11. [Epub ahead of print] PubMed PMID: 20710122.
Rao BS, Mahesh YU, Suman K, Charan KV, Lakshmikantan U, Gibence HR, Shivaji S. Meiotic maturation of vitrified immature chousingha (Tetracerus quadricorni) oocytes recovered postmortem. Cryobiology. 2010 Dec 17. [Epub ahead of print] PubMed PMID: 21168399.
Tachibana M, Sparman M, Mitalipov S. Chromosome transfer in mature oocytes.
Nat Protoc. 2010;5(6):1138-47. Epub 2010 May 27. PubMed PMID: 20539289.
Cervera RP, Martí-Gutiérrez N, Escorihuela E, Moreno R, Stojkovic M. Trichostatin A affects histone acetylation and gene expression in porcine somatic cell nucleus transfer embryos. Theriogenology. 2009 Nov;72(8):1097-110. Epub 2009 Sep 17. PubMed PMID: 19765811.
Chen AE, Egli D, Niakan K, Deng J, Akutsu H, Yamaki M, Cowan C, Fitz-Gerald C, Zhang K, Melton DA, Eggan K. Optimal timing of inner cell mass isolation increases the efficiency of human embryonic stem cell derivation and allows generation of sibling cell lines. Cell Stem Cell. 2009 Feb 6;4(2):103-6. PubMed PMID: 19200798.
Dechiara TM, Poueymirou WT, Auerbach W, Frendewey D, Yancopoulos GD, Valenzuela DM. VelociMouse: fully ES cell-derived F0-generation mice obtained from the injection of ES cells into eight-cell-stage embryos. Methods Mol Biol. 2009;530:311-24. PubMed PMID: 19266341.
Egli D, Sandler VM, Shinohara ML, Cantor H, Eggan K. Reprogramming after chromosome transfer into mouse blastomeres. Curr Biol. 2009 Aug 25;19(16):1403-9. Epub 2009 Aug 13. PubMed PMID: 19682906.
Huang KW. Archiving biomedical mouse models by ovary cryopreservation. 2009. Dissertation Utrecht University, Faculty of Veterinary Medicine. ISBN: 978-90-393-5208-3.
Kobolak J, Kiss K, Polgar Z, Mamo S, Rogel-Gaillard C, Tancos Z, Bock I, Baji AG, Tar K, Pirity MK, Dinnyes A. Promoter analysis of the rabbit POU5F1 gene and its expression in preimplantation stage embryos. BMC Mol Biol. 2009 Sep 4;10:88. PubMed PMID: 19732419; PubMed Central PMCID: PMC2751759. Open Access Download
Sato H, Amagai K, Shimizukawa R, Tamai Y. Stable generation of serum- and feeder-free embryonic stem cell-derived mice with full germline-competency by using a GSK3 specific inhibitor. Genesis. 2009 Jun;47(6):414-22. PubMed PMID: 19391115; PubMed Central PMCID: PMC2726955. Open Access Download
Siripattarapravat K, Pinmee B, Venta PJ, Chang CC, Cibelli JB. Somatic cell nuclear transfer in zebrafish. Nat Methods. 2009 Oct;6(10):733-5. Epub 2009 Aug 30. PubMed PMID: 19718031. View Supplementary Data and Movies
Tachibana M, Sparman M, Sritanaudomchai H, Ma H, Clepper L, Woodward J, Li Y, Ramsey C, Kolotushkina O, Mitalipov S. Mitochondrial gene replacement in primate offspring and embryonic stem cells. Nature. 2009 Sep 17;461(7262):367-72. Epub 2009 Aug 26. PubMed PMID: 19710649; PubMed Central PMCID: PMC2774772. Supplementary Data and Movie
2008
Hsiao EC, Yoshinaga Y, Nguyen TD, Musone SL, Kim JE, Swinton P, Espineda I, Manalac C, deJong PJ, Conklin BR. Marking embryonic stem cells with a 2A self-cleaving peptide: a NKX2-5 emerald GFP BAC reporter. PLoS One. 2008 Jul 2;3(7):e2532. PubMed PMID: 18596956; PubMed Central PMCID: PMC2430532. Open Access Download
Scherzer J, Fayrer-Hosken RA, Ray L, Hurley DJ, Heusner GL. Advancements in large animal embryo transfer and related biotechnologies. Reprod Domest Anim. 2008 Jun;43(3):371-6. Epub 2008 Jan 22. Review. PubMed PMID: 18226021.
Sidhu KS, Walke S, Tuch BE. Derivation and propagation of hESC under a
therapeutic environment. Curr Protoc Stem Cell Biol. 2008 Jul;Chapter 1:Unit 1A.4. PubMed PMID: 18770626.
Yong, H-L. Effect of Making a Hole in Zona Pellucida by Laser on Hatching of Frozen-thawed ICR Mouse Embryos. Korean Journal of Embryo Transfer / v.23, no.1, 2008, pp.1-4. (Abstract in English)
2007
Hall VJ, Compton D, Stojkovic P, Nesbitt M, Herbert M, Murdoch A, Stojkovic M. Developmental competence of human in vitro aged oocytes as host cells for nuclear transfer. Hum Reprod. 2007 Jan;22(1):52-62. Epub 2006 Sep 6. PubMed PMID: 16957049.
Poueymirou WT, Auerbach W, Frendewey D, Hickey JF, Escaravage JM, Esau L, Doré AT, Stevens S, Adams NC, Dominguez MG, Gale NW, Yancopoulos GD, DeChiara TM, Valenzuela DM. F0 generation mice fully derived from gene-targeted embryonic stem cells allowing immediate phenotypic analyses. Nat Biotechnol. 2007 Jan;25(1):91-9. Epub 2006 Dec 24. PubMed PMID: 17187059.
Turetsky T, Aizenman E, Gil Y, Weinberg N, Shufaro Y, Revel A, Laufer N, Simon A, Abeliovich D, Reubinoff BE. Laser-assisted derivation of human embryonic stem cell lines from IVF embryos after preimplantation genetic diagnosis. Hum Reprod. 2008 Jan;23(1):46-53. Epub 2007 Nov 7. PubMed PMID: 17989069.
2006
Tanaka N, Takeuchi T, Neri QV, Sills ES, Palermo GD. Laser-assisted blastocyst dissection and subsequent cultivation of embryonic stem cells in a serum/cell free culture system: applications and preliminary results in a murine model. J Transl Med. 2006 May 8;4:20. PubMed PMID: 16681851; PubMed Central PMCID: PMC1479373. (Download PDF of Full Paper)
2005
Hartshorn C, Anshelevich A, Wangh LJ. Laser zona drilling does not induce hsp70i transcription in blastomeres of eight-cell mouse embryos. Fertil Steril. 2005 Nov;84(5):1547-50. PubMed PMID: 16275269.
Posters and Abstracts Chimera Production by 8-cell Injection (AN 2902 - June 2009)
Ian R Rosewell, Transgenic Services, Cancer Research UK - London Research Institute
Download Application Note
Laser-assisted zona-drilling increased in vitro fertilization with frozen semen in rabbit (Download Abstract)
Varga, E; Polgar, Z ; Bodo, S; Dinnyes, A.
16 th International Congress on Animal Reproduction, July 14-17, 2008
INTRODUCTION: In vitro fertilization allows oocytes to be fertilized in l aboratory conditions with valuable sperm, and can resolve fertility problems. The sperm of valuable males can be cryo-banked. The aim of our study was to increase the fertilization ability of frozen rabbit semen.
CONCLUSION: We determined that the frozen-thawed rabbit semen can fertilize, but penetrates the zona pellucida with lower rates. We report for the first time that application of laser zona drilling in rabbits can increase significantly in vitro fertilization with frozen-thawed rabbit semen. Somatic Cell Nucelar Transfer in Zebrafish (Download Abstract)
Siripattarapravat, Kannika, Venta, Patrick J., Cibelli, Jose B.
6th Annual ISSCR Meeting, June 11-14, 2008, Philadelphia, PA
Poster #217
"Recipient eggs were collected and held in vitro in Chinook salmon ovarian fluid until micromanipulation was completed. While keeping the chorion intact, the fluorescence DNA stained metaphase plate of the egg was ablated using a laser XYClone module [Hamilton Thorne Biosciences, Inc.]. The donor nucleus was then transferred through a micropyle, the natural sperm entry site in the chorion."
Utilization of Laser Assisted Hatching to Improve the Pregnancy Rate of In Vitro Fertilized Bovine Embryos (Download Poster)
S. MengesA, H. WeiB, D. FaberB, D. KraemerA, and C. LongA
ADepartment of Veterinary Physiology and Pharmacology, Texas A&M University, College Station, TX 77843, USA, BTransova Genetics, Sioux Center, IA 51250, USA
"These results demonstrate that laser assisted hatching using the XYClone system does not inhibit in vitro embryo viability and development. A significant difference was found in the 60 day pregnancy rate between control and cut embryos. This difference exhibits a large potential for this system to improve 60 day pregnancy rates of in-vitro produced embryos."
The Effect of Lipid Segregation with or without Zona Pellucida Laser Drilling on Post Thaw Embryo Development of In Vitro Produced Bovine Embryos (Download Poster)
J.H. Pryor1, C.R. Looney1, S. Romo2, D.C. Kraemer3and C.R. Long3
1Ovagenix, Bryan, TX 77805; 2Facultad de Estudios Superiores Cuautitlan, UNAM, Mexico; 3Texas A&M University, Department of Veterinary Physiology and Pharmacology, College Station, TX 77843
The objective of this study was to determine if lipid segregation with or without zona pellucida cutting on post thawed in vitro produced bovine embryos would effect survivability and development.
CBCFLAH and LAH exhibited a significantly greater number of both total and live cells than Control (P<0.05)
Laser Assisted IVF: A Simple Technique to Improve IVF in Mice without Micromanipulation (Download full poster)
Bryan J. Woodward and Keith H.S. Campbell, School of Biosciences, University of Nottingham, Sutton Bonington, Leics, LE12 5RD UK
A new approach to cyropreservation of large equine embryos by vitrification after blastocoel micromanipulation. (Download full PDF Abstract )
J. Scherzer, R. A. Fayrer-Hosken, L. Ray and G. Heusner
Reproduction, Fertility and Development 19(1) 225 - 226 (abstracts from IETS 2007)
SUMMARY (EXCERPT)
Vitrified large equine embryos >800 µm recovered on Day 8 after ovulation have not been successfully transferred yet. In this study, we examined the effects of reduction of the blastocoelic fluid and microinfusion of a cryopreservative prior to vitrification on pregnancy outcome. To facilitate aspiration and microinfusion, a laser system (XYclone; Hamilton Thorne, Beverly, MA, USA) was applied. Approximately 20% of the total blastocoelic fluid was removed using a standard micromanipulator and microcapillary system (Eppendorf). All embryos were then vitrified as previously described (Eldridge-Panuska et al. 2005 Theriogenology 63, 1308-1319).
None of the control embryos nor the split embryo in the treatment group led to the formation of an embryonic vesicle. However, the blastocyst, which had undergone both aspiration of blastocoelic fluid and microinfusion of VS1 (#5), had formed an embryonic vesicle at Day 15 after ovulation. During a further exam on Day 28, the uterine tone in the recipient was still increased, but ultrasonography revealed resorption of the embryo, which was probably caused by heat stress. Nevertheless, we will test this protocol for the cryopreservation of large equine embryos on a larger scale during the next breeding season.
Effects of Caffeine on the In Vitro Development of Bovine Nuclear Transfer Embryos. Maalouf WE, Lee J-H, Campbell KHS. Animal Development and Biotechnology Group, School of Biosciences, University of Nottingham. Presented at IETS 2006. (View Poster PDF)
A New Method to Produce Mice Fully Derived from Embryonic Stem Cells. William T. Poueymirou, Wojtek Auerbach, Joseph Hickey, Jennifer Escaravage, David Frendewey, Niels C. Adams, George D. Yancopoulos, Thomad D. DeChiara, and David M. Valenzuela. Regerneron Pharmaceuticals, Inc. 777 Old Saw Mill River Road., tarrytown, NY, USA. Presented at the 2005 Transgenic Technology Meeting, Barcelona, Spain, September 11-13, 2005.(View the Full Poster PDF)
A Feeder Cell- and Serum-Free System to Harvest Embryonic Stem Cells From Laser Dissected Blastocysts. T. Takeuchi, N. Tanaka, Q.V. Neri, Z. Rosenwaks, G.D. Palermo. Cornell University, New York, NY. Abstract from American Society of Reproductive Medicine, September 2005 (View Abstract PDF)
Laser zona drilling does not induce hsp70i transcription in blastomeres of eight-cell mouse embryos. Cristina Hartshorn, Ph.D., Aleksandra Anshelevich, B.S.,
Lawrence J. Wangh, Ph.D.
To assess whether zona drilling with a 1,480-nm laser induces heat shock in eight-cell embryos, we measured hsp70i RNA levels in sets of single blastomeres isolated after laser treatment of mouse embryos that had or had not been heated at 43°C. Unlike heating, laser zona drilling did not stimulate hsp70i expression, even in the blastomeres closest to the laser beam, corroborating the safety of this procedure for assisted reproduction.
(Fertil Steril 2005;84:1547–50. ©2005 by American Society for Reproductive Medicine.)
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