XYClone is for non-clinical research use only in USA. hESC research using the XYClone should be conducted in accordance with any applicable Federal, State or local laws and regulations.
Please Note: The XYClone™ is for use ONLY with ANIMAL and STEM CELL APPLICATIONS. (For Clinical Laser Assisted Hatching and Biopsy applications, please see the ZILOS-tk.)

XYClone for Laser-assisted IVF

"A main criteria for successful IVF is the use of sperm with acceptable motility, concentration, and forward movement. If one or more of these parameters is suboptimal, the ability to fertilize oocytes can be severely limited. In these cases, an XYClone™ laser is used to create 2-3 holes in the zona pellucida of each oocyte providing a means for less motile or less progressive sperm to penetrate the oocyte and induce fertilization. This procedure has greatly enhanced the fertilization efficiency of various genetically-modified lines and is a vital tool in the rescue of valuable rodent strains." Charles River Laboratories

• Benefits of Laser-assisted IVF
• Laser-assisted IVF: A Simple Technique to Improve IVF in Mice without Micromanipulation
• Laser-assisted IVF Fertilization Percents

Benefits of Laser-assisted IVF

• Increase fertilization rates with mutant and low motility sperm
• Increase overall throughput and production
• Significantly reduces the need for ICSI

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Laser Assisted IVF: A Simple Technique to Improve IVF in Mice without Micromanipulation (Download full poster)

CONCLUSIONS

• Zona laser dissection of mouse oocytes significantly improved the fertilization rate following IVF.
• This simple swift technique avoided micromanipulation and problems associated with ICSI. Furthermore, the reduced operator time allowed numerous oocytes to be treated in a short period.

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Laser-assisted IVF Fertilization Percents (Download full PPT as PDF)

Kathy Mohr, Mutant Mouse Regional Resource Center, University of North Carolina

Fresh sperm (wild type & mutant)
C57BL/6 sperm + CD-1 oocytes

Using Thawed Sperm: C57BL/6 sperm + oocytes

There was normal development to blast and onward to hatching using XYClone laser for assisted IVF. Oocytes undergoing laser assisted IVF hatched 8-12 hours sooner than conventional IVF.

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