XYClone is for non-clinical research use only in USA. hESC research using the XYClone should be conducted in accordance with any applicable Federal, State or local laws and regulations.
Please Note: The XYClone™ is for use ONLY with ANIMAL and STEM CELL APPLICATIONS. (For Clinical Laser Assisted Hatching and Biopsy applications, please see the ZILOS-tk.)

XYClone for Laser-assisted IVF

• Benefits of Laser-assisted IVF
• Laser-assisted IVF: A Simple Technique to Improve IVF in Mice without Micromanipulation
• Laser-assisted IVF Fertilization Percents

Benefits of Laser-assisted IVF

• Increase fertilization rates with mutant and low motility sperm
• Increase overall throughput and production
• Significantly reduces the need for ICSI

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Laser Assisted IVF: A Simple Technique to Improve IVF in Mice without Micromanipulation (Download full poster)

CONCLUSIONS

• Zona laser dissection of mouse oocytes significantly improved the fertilization rate following IVF.
• This simple swift technique avoided micromanipulation and problems associated with ICSI. Furthermore, the reduced operator time allowed numerous oocytes to be treated in a short period.

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Laser-assisted IVF Fertilization Percents (Download full PPT as PDF)

Kathy Mohr, Mutant Mouse Regional Resource Center, University of North Carolina

Fresh sperm (wild type & mutant)
C57BL/6 sperm + CD-1 oocytes

Using Thawed Sperm: C57BL/6 sperm + oocytes

There was normal development to blast and onward to hatching using XYClone laser for assisted IVF. Oocytes undergoing laser assisted IVF hatched 8-12 hours sooner than conventional IVF.

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