Stiletto is for non-clinical research use only. hESC research using the Stiletto should be conducted in accordance with any applicable Federal, State or local laws and regulations. (For Clinical Laser Assisted Hatching and Biopsy applications, please see the ZILOS-tk.)

Components of Stiletto™ Laser

• Laser Module and Controller
• Stiletto Software
• Motorized Stage with Plate Holder
• Digital Color Camera & C-Mount Adapter
• Turret Adapters and Hex Key
• High Performance Computer System
• Operators Manual & Help File
• Installation & Training

Stiletto Laser Module & Controller

STILETTO Laser Module: 20x Objective and Class 1, infrared wavelength, 300 mW Laser combined into a single compact turret mounted unit. The Stiletto laser module installs on all current and older models of inverted microscopes. The LWD 20x objective is specially designed and manufactured according to high quality HT specifications. It is constructed to have high transmission in the infrared, as well as in the visible.

Laser Controller: High speed micro controller with instantaneous closed loop laser power feedback control.

Stiletto Software

Laser Control

• Multi-mode options with user-defined parameters
• Manual or automated laser operation
• Manual or automated X-Y stage movement
• Adjustable laser pulse length: 100 µs - 3000 µs
• Adjustable pulse/second
• Adjustable maximum duration
• Computer-controlled target alignment
• Laser firing by Mouse or by optional Remote Fire Foot Switch

Ablation / Scoring Control

• Freehand selection of target areas using mouse
• Select multiple target areas
• Laser application to perimeter of selected area (uniform or variable perimeter)
• X-Y laser crosshatching for ablation or scoring
• Minimum X-Y crosshatch width: 1 µm apart
• Maximum X-Y crosshatch width: 10000 µm apart

Image Acquisition

• Capture of static images (tif, bmp or jpg)
• Manual and automatic image naming
• Image zoom and pan
• Manual and automatic image labeling
• Graphic overlays
• Measurement tool
• Image thumbnail gallery

Video Acquisition

• Used for capture and playback of real-time video (up to 1000 seconds) and time-lapse video (user defined capture interval and playback speed)

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Motorized Stage with Plate Holder

• X-Y motorized stage: [Prior Scientific, ProScan] Customized for make and model specific inverted microscope
• Microtitre plate holder for motorized stage: [Prior H223RNC] 85x128mm, recessed, for well plates with chamfered corners. Compatible with most 6-, 24-, 96- and 384-well plates.

Color Digital Camera and C-Mount Adapter

Digital (USB 2.0) color camera (max resolution 1392 x 1040). All Stiletto systems also come with the appropriate c-mount adapter for your microscope.

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Turret Adapters and Hex Key

A set of four turret adapters is included with each Stiletto. These adapters allow you install the Stiletto on any inverted microscope.

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High Performance Computer System

Desktop computer including digital image capture board, all required cables, DVD-RW, mouse and keyboard. A 20 inch, flat screen monitor provides clear visualization of embryo and saves space on the workbench.

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Operator's Manual and Windows-based Help System

A clear and concise printed manual (in color) is included with the Stiletto. In addition, a comprehensive Windows Help file is installed.

Installation and Training

All systems include installation and training by a certified Hamilton Thorne distributor, trained specifically in the installation and operation of the Stiletto system.

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