Stiletto is for non-clinical research use only. hESC research using the Stiletto should be conducted in accordance with any applicable Federal, State or local laws and regulations. (For Clinical Laser Assisted Hatching and Biopsy applications, please see the ZILOS-tk.)

Applications of Stiletto Laser

• Cell Ablation
• Colony Scoring
• Cell Selection

Cell Ablation

By applying the laser pulses within the selected areas, unwanted cell areas are quickly obliterated. The Stilleto allows you to designate multiple areas targeted for abaltion. The motorized stage automatically moves from area to area and the laser is applied to ablate the unwanted cells.

BEFORE ABLATION WITH STILETTO	AFTER ABALTION WITH STILETTO

The video below is a simulation on ink to demonstrate the speed and precision of the Stiletto laser.

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Colony Scoring

By applying the laser pulses in a crosshatch pattern within the selected areas, cell colonies are quickly segmented for propogation. Users may designated the size of resulting sections. The image belows shows the scoring of a stem cell colony using the Stiletto.
Left to right: 100 µm x 150 µm, 200 µm x 300 µm, 400 µm x 600 µm

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Cell Selection

By applying the laser to only the perimeter of selected areas, specific cell populations can be isolated.

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