Hamilton Thorne is pleased to introduce the MouseTraxx sperm analysis system - configured specifically for the TRANSGENIC MOUSE facility. Whether analyzing fresh or frozen/thawed sperm, MouseTraxx provides fast, accurate and objective results.

 

Features

• Ease of Use
• Results You Need
• Real-Time Quality Control
• Summary of Sperm Analysis Results Provided
• Components
• Included Options & Accessories

 

The MouseTraxx hardware and software have been designed to provide users with a system that offers high-end performance while maintaining its ease of use. The intuitive Windows-based software promotes fast learning and quickly increases the confidence level of users. With a minimum investment of time, even users unfamiliar with computers will be performing fast, accurate and reliable sperm analyses. You don’t need to be a computer expert to analyze sperm with the MouseTraxx.

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Results You Need

Whether you need basic counts and motilities or detailed analysis of sperm motion, the MouseTraxx CASA system provides you with the results you need. Results for motile, progressively motile and static sperm include actual number counted, sample total, concentrations and percentages. Additional results include averages and distribution bar charts of velocities, motion characteristic and morphometry.

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Real-Time Quality Control

MouseTraxx provides on demand quality control with the Playback feature. Using image Playback, the accuracy of the analysis is confirmed. To optimize the system, analysis parameters may be adjusted using the two interactive QC Plots or directly from the analysis setup screen.

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Summary of Sperm Analysis Results Provided

Counts:

Total, Motile, Progressive

% Motile, % Progressively Motile

Rapid, Medium, Slow and Static Cells

Concentrations

Total, Motile, Progressive (millions/ml)

Rapid, Medium, Slow and Static Cells (millions/ml)

Mean Values

VAP: Smoothed Path Velocity (microns/sec)

VCL: Track Velocity (microns/sec)

VSL: Straight Line Velocity (microns/sec)

ALH: Amplitude of Lateral Head Displacement (microns)

BCF: Beat Cross Frequency (hertz)

LIN: Linearity (ratio of VSL/VCL)

STR: Straightness (ratio of VSL/VAP)

Elongation: head shape (ratio of minor to major axis of sperm head)

Area: head size (square microns)

Bar Chart Distributions

VAP, VCL, VSL, Elongation, ALH, BCF, LIN, STR

 

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Components

MouseTraxx Computer

• Desktop computer system running Windows XP (see specification sheet for hardware details)
DVD-RW drive
• 20" flat panel monitor
• Security key
• Keyboard and Mouse

Microscope and Camera

• Olympus CX41 Microscope with Trinocular Head
• 4x Dark Field Objective
• 10x Negative Phase Objective
• 60 Hz RS-170 Camera (provides capability to read/record in NTSC format)
• MiniTherm Stage Warmer (Part # 720230)

Animal Motility Software

• Percents, Counts and Concentrations of total, motile, progressive and static cells
• Velocities: Average Path (VAP), Curvilinear (VCL), Straight line (VSL)
• Motion Parameters: Linearity (LIN), Straightness (STR), Beat Cross Frequency (BCF), Amplitude of Lateral Head Displacement (ALH)
• Morphometric Parameters: Head Size, Elongation
• 4 Level Quality Control including Playback and QC Plots (Size, Intensity, Elongation)

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Included Options & Accessories

HDATA ASCII Export : Provides capability for export of delimited ASCII data files of all calculated and manually-entered data to external programs, spreadsheets, database, etc. External programs not included. (more information)

Accu-beads: (one box) Used for quality control. Each box includes one 5 ml bottle each of 18 M/ml and 35 M/ml concentrations (more information)

2X-CEL Slides: One box each of 20 µm and 80 µm analysis slides (more information)

Leja Slides: One box of 100 µm fixed coverslip slides.