Since sperm motility and velocity are highly dependent on temperature, assessment of motility should be performed as close to 37°C as possible [World Health Organization laboratory manual for the examination of human semen. 3rd edition. 1992].
Before, large and costly microscope stage heaters were the only option. Now, with the MiniTherm stage warmer, a portable, low-cost alternative is available. Designed for use with disposable sperm analysis chambers and microscope slides, the MiniTherm stage warmer maintains sample temperature at 37°C. Simply plug the warmer in and place on the microscope stage. The MiniTherm stage warmer automatically calibrates itself to 37°C. Each MiniTherm comes with a temperature validation slide that turns green (see image above) when at 37°C.