What Are Accu-Beads+?

Accu-Beads+ consist of two known concentrations of latex beads (18 and 35 M/ml) suspended in an aqueous solution. Similar in size to human sperm, the Accu-Beads+ have been developed to confirm accurate counting procedures used for routine semen analysis.

Who Will Benefit from Using Accu-Beads+?

Whether you perform your semen counts manually or using an automated analyzer, you can use Accu-Beads+ for quality control.

What do Accu-Beads+ Evaluate?

For manual methods, the Accu-Beads+ are used to evaluate the actual counting procedures practiced. When used in an automated semen analyzer, the Accu-Beads+ test and evaluate the optical and image acquisition systems of the analyzer. Because motility is irrelevant for checking these functions, the static nature of the Accu-Beads+ will not affect the quality control testing.

How are Accu-Beads+ used?

Using a pipette, simply draw the required amount (undiluted) and load your standard semen analysis chamber, such as a disposable slide or a Petroff-Hausser, Makler, or Hemacytometer chamber. Position the chamber on the stage of the microscope or analyzer and perform an analysis according to your normal procedure.


The images below show the 35 M/ml Accu-Beads+ in the IVOS analyzer. The top image shows the Accu-Bead image acquired for analysis and the second shows the analyzed Accu-Bead image.

Additional Information

Accu-Beads+ are packaged in 5 ml aliquots and are stable at room temperature for up to 2 years. Complete instructions for use with the Hamilton Thorne Research automated analyzers and for manual counting methods are included with each package.

FileDescriptionFile size
Download this file (710110-accu-beads+Instructions-2015-April-20-RevD.pdf)Accu-Beads+ Instruction SheetAccu-Beads+ Instruction Sheet (Rev. D. April 20, 2015)455 kB
Download this file (Accubeads+ Suspension MSDS-27-October-2014.pdf)Accubeads+ Suspension MSDS-27-October-2014.pdfAccu-Beads+ Material Safety Data Sheet539 kB
Download this file (AccubeadTechNote.pdf)Accu-beads Technical Note.pdfAccu-beads Technical Note201 kB