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What are accu-beads+?
Accu-beads+ consist of two known concentrations of latex beads (18 and 35 M/ml) suspended in an aqueous solution. Similar in size to human sperm, the accu-beads+ have been developed to confirm accurate counting procedures used for routine semen analysis.
Who will benefit from using accu-beads+?
Whether you perform your semen counts manually or using an automated analyzer, you can use accu-beads+ for quality control.
What do accu-beads+ evaluate?
For manual methods, the accu-beads+ are used to evaluate the actual counting procedures practiced. When used in an automated semen analyzer, the accu-beads+ test and evaluate the optical and image acquisition systems of the analyzer. Because motility is irrelevant for checking these functions, the static nature of the accu-beads+ will not affect the quality control testing.
How are accu-beads+ used?
Using a pipette, simply draw the required amount (undiluted) and load your standard semen analysis chamber, such as a disposable slide or a Petroff-Hausser, Makler, or Hemacytometer chamber. Position the chamber on the stage of the microscope or analyzer and perform an analysis according to your normal procedure.
The images below show the 35 M/ml accu-beads+ in the IVOS analyzer. The top image shows the accu-beads + image acquired for analysis and the second shows the analyzed accu-beads + image.
Accu-beads+ are packaged in 5 ml aliquots and are stable at room temperature for up to 2 years. Complete instructions for use with the Hamilton Thorne Research automated analyzers and for manual counting methods are included with each package.