Variability in chamber depth is the single greatest source of error in calculating concentrations and total counts. The 2X-CEL chamber is subjected to stringent QC testing using our patented "Interferometer" to measure precision of chamber depth. Chamber materials have also been scientifically tested for sperm toxicity and viability in the HT laboratories.
To accommodate all sample types, the 2X-CEL chambers are easily loaded by either the capillary action or drop method. The 2X-CEL chambers are used with the HTR Magnetic Slide Holder, an easy to load system that evenly distributes pressure along the edges of the coverslip to achieve precise chamber depths. Because the coverslip of the 2X-CEL is not permanently mounted on the slide, the chamber may be easily cleaned to promote detritus-free analysis. The 2X-CEL chambers may also be used with the MiniTherm Slide Warmer. For added efficiency, each slide accommodates two samples.
The 20 micron 2X-CEL sperm analysis chamber is designed with a special hydrophobic barrier to guard against overfilling, resulting in increased accuracy in determination of sperm concentrations and total counts. Developed specifically for motility analysis of rat sperm, the 80 micron 2X-CEL provides an easy to handle, clean alternative to the cannula.
Please note that there are different thicknesses of coverslips for each type of chamber. Coverslips cannot be used interchangeably.