IDENT STAIN SEQUENTIAL DILUTIONS CALCULATOR
(based on Total Parts Method)

Step I: Determine A
(1st Dilution - Raw Sample to Diluent)

Step II: Determine B
(2nd Dilution - Sample to IDENT Stain)

Step III: Determine D
( Final Dilution Ratio to be entered into IVOS)

Only the values in red need to be entered.

STEP I : Basic Sample Dilution
Value
Parts Raw Sample (always 1)
X1
1
Enter Parts Diluent
X2
STEP II : IDENT Stain Dilution
Value
Parts Diluted Semen Sample (always 1)
Y1
1
Enter Parts IDENT Stain Solution
Y2
CLICK BUTTON TO CALCULATE FINAL DILUTION
Intermediate Calculation of A
X1 + X2
Intermediate Calculation of B
Y1 + Y2
Intermediate Calculation of AB AB
Calculation of Final Dilution D
(AB) - 1